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Objective To study the repairment of pancreas mesenchymal stem cells(MSCs) on injured pancreas of rats,and find a new source of cells for treatment of diabetes. Methods ① Pancreases were taken out of three-day-old Wistar rats under bioclean condition,and cells were obtained by V-collagenase digestion. Generations were passed by conventional culture,cells were purified by adherence screening method,cell morphous was observed by Giemsa staining. The expressions of surface marker CD34 and CD44 were determined by FCM,and compared with bone marrow MSCs(BM-MSCs),the differences in character and function were observed. ②The experimental rats were divided into two groups randomly. Pancreas ischemic necrosis model was made by deligation,then the purified pancreas MSCs were marked with DAPI and then were transplantated partially. After two weeks,the survival rate was measured and histopathological detection was performed. Results ① The cells had concordant morphous gradually with vigorous generation. There was no significant difference in morphology and surface antigen compared with BM-MSCs.② The survival rate in experimental group was 75% ,the necrotic tissue had basi-rebounded. Blue fluorescent was observed in repaired pancreas tissues. The survival rate in control group was 20%. The survival rate in experimental group was higher than that in control group (P
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0.999 9).The minimum quantitative concentrations were 0.027 ?g?mL-1,0.033 ?g?mL-1,0.095 ?g?mL-1,respectively.The 3 kinds of organic solvents were not found in each batch except 0.10% and 0.15% ethyl acetate was found in 2 batches of samples.CONCLUSIONS:The established method is accurate,sensitive and accurate for the determination of 3 kinds of residual organic solvents in beclometasone dipropionate simultaneously.